BET BIOPETRO

TECHNICAL PRODUCT BULLETIN #B-48
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: NOVEMBER 10, 1993
REVISED LISTING DATE: AUGUST 31, 2000
"BET BIOPETRO"
LAST COMMUNICATION WITH MANUFACTURER: 05/18/2006
LAST ATTEMPT: 8/11/2021

I. NAME, BRAND, OR TRADEMARK
BET BIOPETRO Type of Product: Biological Additive

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
BioEnviroTech ADDRESS: updated information required PHONE: updated information required (Mr. Warren Butler)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
BioEnviroTech ADDRESS: updated information required PHONE: updated information required (Mr. Warren Butler)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-Flammable 2. Ventilation: Normal ventilation 3. Skin and eye contact; protective clothing; treatment in case of contact: Avoid eye and skin contact. In case of eye contact, flush eyes with water or eyewash, and refrain from rubbing. For skin contact, wash with mild soap and apply hand cream if itching or redness occurs. Avoid inhalation. In case of inhalation, seek fresh air. Repeated inhalation has been associated with respiratory allergy in some persons. Such allergic individuals should wear protective clothing and eye goggles. In enclosed buildings, workers should wear single use nuisance dust masks appropriate for fine particulate dust. In outdoor field applications, no dust mask is required. 4.a. Maximum storage temperature: 105ºF 4.b. Minimum storage temperature: 32ºF 4.c. Optimum storage temperature range: 50ºF to 70ºF 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-Flammable
2. Ventilation:
Normal ventilation
3. Skin and eye contact; protective clothing; treatment in case of contact:
Avoid eye and skin contact. In case of eye contact, flush eyes with water or eyewash, and refrain from rubbing. For skin contact, wash with mild soap and apply hand cream if itching or redness occurs. Avoid inhalation. In case of inhalation, seek fresh air. Repeated inhalation has been associated with respiratory allergy in some persons. Such allergic individuals should wear protective clothing and eye goggles. In enclosed buildings, workers should wear single use nuisance dust masks appropriate for fine particulate dust. In outdoor field applications, no dust mask is required.
4.a. Maximum storage temperature: 105ºF
4.b. Minimum storage temperature: 32ºF
4.c. Optimum storage temperature range: 50ºF to 70ºF
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
More than 3 years in unopened original shipping container, stored in cool dry area.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: BET BIOPETRO is a powder containing granules of bacterial product formulated to provide performance in the bioremediation of heavy refined and crude hydrocarbon contaminants in both soil and water environments. Application procedure and treatment schedule will vary with specific environmental conditions and bioremediation requirements. Contact BET for specific technical advice. 2. Concentration/Application Rate: Dosage will vary with specific environmental conditions and bioremediation requirements. Contact BET for specific technical advice. 3. Conditions for Use: BET BIOPETRO should be used at temperatures in the range of 45ºF to 100ºF, pH range of 5.5 to 8.5, and dissolved oxygen level of 3 to 5 mg/l. The product is effective in both salt and fresh waters. However, where extreme salinity is projected, testing and evaluation of the bacteria in bioremediation are advised. BET BIOPETRO cultures exhibit some resistance to toxic shocks from strong chemicals. Nonetheless, highly chlorinated compounds, acids, caustics, disinfectants, germicides, and chlorine will render the cultures ineffective, just like other bacteria. Where such chemicals are anticipated, testing and evaluation of the bacteria in bioremediation are advised. BET BIOPETRO cultures require supplemental nutrients for optimum performance (See section VIII. 4.).

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
BET BIOPETRO is a powder containing granules of bacterial product formulated to provide performance in the bioremediation of heavy refined and crude hydrocarbon contaminants in both soil and water environments.
Application procedure and treatment schedule will vary with specific environmental conditions and bioremediation requirements. Contact BET for specific technical advice.
2. Concentration/Application Rate:
Dosage will vary with specific environmental conditions and bioremediation requirements. Contact BET for specific technical advice.
3. Conditions for Use:
BET BIOPETRO should be used at temperatures in the range of 45ºF to 100ºF, pH range of 5.5 to 8.5, and dissolved oxygen level of 3 to 5 mg/l. The product is effective in both salt and fresh waters. However, where extreme salinity is projected, testing and evaluation of the bacteria in bioremediation are advised. BET BIOPETRO cultures exhibit some resistance to toxic shocks from strong chemicals. Nonetheless, highly chlorinated compounds, acids, caustics, disinfectants, germicides, and chlorine will render the cultures ineffective, just like other bacteria. Where such chemicals are anticipated, testing and evaluation of the bacteria in bioremediation are advised. BET BIOPETRO cultures require supplemental nutrients for optimum performance (See section VIII. 4.).

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT BET BIOPETRO	29965.2 31494.8 25914.5	0 0 0	5620.5 6660.2 5569.1	0 0 0
7	CONTROL NUTRIENT BET BIOPETRO	30101.5 073.9 373.0	0 0 0	5610.1 5086.6 1220.0	0 0 0
28	CONTROL NUTRIENT BET BIOPETRO	28785.6 706.2 350.0	4.0 98.0 99.0	5512.7 4863.7 1882.9	2.0 27.0 67.0

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 51%
Product (BET BIOPETRO): 30%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 6.6 to 7.4 Temperature: 70ºF to 95ºF Salinity: Fresh to salt water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: < 5.5 and > 8.5 Temperature: < 45ºF and > 105ºF Salinity: extreme salinity 4. Special nutrient requirements: BioEnviroTech, Inc. recommends nutrient supplements for optimum performance of the product in bioremediation application. The required nutrients are dependent upon hydrocarbon contamination levels. Contact BET for specific nutrient dosing requirements. 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6.6 to 7.4
Temperature: 70ºF to 95ºF
Salinity: Fresh to salt water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: < 5.5 and > 8.5
Temperature: < 45ºF and > 105ºF
Salinity: extreme salinity
4. Special nutrient requirements:
BioEnviroTech, Inc. recommends nutrient supplements for optimum performance of the product in bioremediation application. The required nutrients are dependent upon hydrocarbon contamination levels. Contact BET for specific nutrient dosing requirements.
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

BET BIOPETRO

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