ESAM Collaborative Analytical Methods and Protocols for Chemistry

EPA's Homeland Security Research Program (HSRP) has worked with partnering laboratories and agencies to develop several of the analytical methods and protocols addressing target chemical, radiochemical, pathogen and biotoxin contaminants of concern in environmental samples, as well as radiochemical contamination of outdoor building and infrastructure materials. 

The methods developed by HSRP are a subset of the methods included in EPA's SAM 2022 and can also be accessed here.

Chemistry Methods

• U.S. EPA and CDC. 2011. "Surface Analysis Using Wipes for the Determination of Nitrogen Mustard Degradation Products by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)." Cincinnati, OH: EPA. EPA/600/R-11/143.
• U.S. EPA and CDC. 2013. "Surface Analysis of Nerve Agent Degradation Products by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)." Cincinnati, OH: U.S. EPA. EPA/600/R-13/224. 
• U.S. EPA. 2013. "Verification of Methods for Selected Chemical Warfare Agents (CWAs)." Washington, DC: U.S. EPA. EPA/600/R-12/653.
• U.S. EPA. 2015. "Extraction and Analysis of Lewisite 1, by its Degradation Products, Using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)," Revision 1. Washington, DC: U.S. EPA. EPA/600/R-15/258.
• U.S. EPA. 2016. "Adaptation of the Conditions of U.S. EPA Method 538 for the Analysis of a Toxic Degradation Product of Nerve Agent VX (EA2192) in Water by Direct Aqueous Injection- Liquid Chromatography/Tandem Mass Spectrometry." Cincinnati, OH: U.S. EPA. EPA/600/R-15/097.
• U.S. EPA. 2016. "Analytical Protocol for VX Using Gas Chromatography/Mass Spectrometry (GC/MS)." Cincinnati, OH: U.S. EPA. EPA/600/R-16/116.
• U.S. EPA. 2016. "Analytical Protocol for Cyclohexyl Sarin, Sarin, Soman and Sulfur Mustard Using Gas Chromatography/Mass Spectrometry." Cincinnati, OH: U.S. EPA. EPA/600/R-16/115. 
• U.S. EPA. 2016. "Analytical Protocol for Measurement of Extractable Semivolatile Organic Compounds Using Gas Chromatography/Mass Spectrometry." Cincinnati, OH: U.S. EPA. EPA/600/R-16/114. 
• ASTM. 2016. "Method D7597-16: Standard Test Method for Determination of Diisopropyl Methylphosphonate, Ethyl Hydrogen Dimethylamidophosphate, Ethyl Methylphosphonic Acid, Isopropyl Methylphosphonic Acid, Methylphosphonic Acid and Pinacolyl Methylphosphonic Acid in Water by Liquid Chromatography/Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method D7598-16: Standard Test Method for Determination of Thiodiglycol in Water by Single Reaction Monitoring Liquid Chromatography/Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method D7599-16: Standard Test Method for Determination of Diethanolamine, Triethanolamine, N-Methyldiethanolamine and N-Ethyldiethanolamine in Water by Single Reaction Monitoring Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method D7644-16: Standard Test Method for Determination of Bromadiolone, Brodifacoum, Diphacinone and Warfarin in Water by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method D7645-16: Standard Test Method for Determination of Aldicarb, Aldicarb Sulfone, Aldicarb Sulfoxide, Carbofuran, Methomyl, Oxamyl and Thiofanox in Water by Liquid Chromatography/Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method E2787-11: Standard Test Method for Determination of Thiodiglycol in Soil Using Pressurized Fluid Extraction Followed by Single Reaction Monitoring Liquid Chromatography/ Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method E2838-11: Standard Test Method for Determination of Thiodiglycol on Wipes by Solvent Extraction Followed by Liquid Chromatography/Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.
• ASTM. 2016. "Method E2866-12: Standard Test Method for Determination of Diisopropyl Methylphosphonate, Ethyl Methylphosphonic Acid, Isopropyl Methylphosphonic Acid, Methylphosphonic Acid and Pinacolyl Methylphosphonic Acid in Soil by Pressurized Fluid Extraction and Analyzed by Liquid Chromatography/Tandem Mass Spectrometry." West Conshohocken, PA: ASTM International.