Rodenticide Study Protocols

EPA has provided the following 26 rodenticide study protocols to evaluate studies submitted by rodenticide manufacturers to support new rodenticide baits and tracking powders containing registered active ingredients for use against mice and rats. These only include lab protocols for conducting laboratory efficacy tests, even though the registrant can conduct and submit to EPA other types of tests to support rodenticide efficacy.

The last three study protocols are for evaluating tamper-resistant bait stations sold in consumer rodenticide products. 

• Laboratory Test Method: 1.201 - Standard Norway Rat and Roof Rat Anticoagulant Liquid Bait (pdf) (920.72 KB)
• Laboratory Test Method: 1.202 - Standard House Mouse Anticoagulant Liquid Bait (pdf) (905.6 KB)
• Laboratory Test Method: 1.203 - Standard Norway Rat/Roof Rat Anticoagulant Dry Bait (pdf) (973.78 KB)
• Laboratory Test Method: 1.204 - Standard House Mouse Anticoagulant Dry Bait (pdf) (980.1 KB)
• Laboratory Test Method: 1.205 - Standard Norway Rat/Roof Rat Anticoagulant Tracking Powder Efficacy (pdf) (899.65 KB)
• Laboratory Test Method: 1.207 - Standard Norway Rat/Roof Rat Acute Liquid Bait (pdf) (883.21 KB)
• Laboratory Test Method: 1.208 - Standard House Mouse Acute Liquid Bait (pdf) (933.15 KB)
• Laboratory Test Method: 1.209 - Standard Norway Rat/Roof Rat Acute Dry Bait (pdf) (968.77 KB)
• Laboratory Test Method: 1.210 - Standard House Mouse Acute Dry Bait (pdf) (1 MB)
• Laboratory Test Method: 1.211 - Standard Norway Rat/Roof Rat Acute Tracking Powder Efficacy (pdf) (897.57 KB)
• Laboratory Test Method: 1.212 - Standard House Mouse Anticoagulant Tracking Powder Efficacy (pdf) (897 KB)
• Laboratory Test Method: 1.213 - Standard Norway Rat/Roof Rat Anticoagulant Wax Block and Wax Pellet (pdf) (995.15 KB)
• Laboratory Test Method: 1.214 - Standard House Mouse Anticoagulant Wax Block and Wax Pellet Laboratory Test Method (pdf) (1010.32 KB)
• Laboratory Test Method: 1.215 - Standard Peromyscus Species Acute Dry Bait (pdf) (1.07 MB)
• Laboratory Test Method: 1.216 - Standard Peromyscus Species Anticoagulant Dry Bait (pdf) (940.14 KB)
• Laboratory Test Method: 1.217 - Standard Norway Rat and Roof Anticoagulant Placepack Dry (pdf) (883.38 KB)
• Laboratory Test Method: 1.218 - Standard House Mouse Anticoagulant Placepack Penetration (pdf) (967.47 KB)
• Laboratory Test Method: 1.219 Standard Norway Rat and Roof Rat Acute Placepack Dry Bait (pdf) (958.18 KB)
• Laboratory Test Method:1.220 - Standard Acute Placepack Dry Bait (pdf) (1005.56 KB)
• Laboratory Test Method: 1.221 - Standard Norway Rat/Roof Rat Anticoagulant Technical and Concentrated Dry Bait (pdf) (1.01 MB)
• Laboratory Test Method: 1.222 - Standard Norway Rat/Roof Rat Acute Technical and Concentrated Dry Bait (pdf) (1013.93 KB)
• Laboratory Test Method: 1.223 - Standard Peromyscus Species Anticoagulant Technical and Concentrated Dry Bait (pdf) (1 MB)
• Laboratory Test Method: 1.224 - Standard Peromyscus Species Acute Technical and Concentrated Dry Bait (pdf) (998.47 KB)
• Laboratory Test Method: 1.225 - Standard House Mouse Anticoagulant Technical and Concentrated Dry Bait (pdf) (1.03 MB)
• Laboratory Test Method: 1.226 - Standard House Mouse Acute Technical and Concentrated Dry Bait (pdf) (1014.12 KB, 5/6/1976)
• Laboratory Test Method: 1.227 - Standard House Mouse Acute Tracking Powder Efficacy (pdf) (883.5 KB)
• Protocol 1.228 - Method for Testing Ready-To-Use Bait Stations with Adults for Facility of Opening, Reclosing, and Securing (pdf) (3.49 MB)
• Protocol 1.229 - Method for Testing Ready-To-Use Bait Stations with Young Children (pdf) (2.68 MB)
• Protocol 1.230 - Method for Testing Ready-To-Use Bait Stations with Dogs (pdf) (2.09 MB)